facsmax cell dissociation solution (AMS Biotechnology)

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AMS Biotechnology facsmax cell dissociation solution
Facsmax Cell Dissociation Solution, supplied by AMS Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/facsmax/FACSmax+Cell+Dissociation+Solution/pmc13010115-14-0-5
Average 96 stars, based on 47 article reviews

facsmax cell dissociation solution - by Bioz Stars, 2026-09

96/100 stars

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Incubation:

Article Title: Single cell transcriptome profiling of the human developing spinal cord reveals a conserved genetic programme with human specific features
Article Snippet: .. Dissected samples were snipped into smaller pieces and incubated on a FACSmax (Amsbio, T200100) cell dissociation solution containing 10X Papain (30 U/mg, Sigma-Aldrich, 10108014001) for 11 min at 37°C to dissociate the cells and transferred to a HBSS solution with 5% FBS, Rock inhibitor (10 μM, Stemcell Technologies, Y-27632) and 1X of non-essential aminoacids (Thermo Fisher Scientific, 11140035). ..

Article Title: Distinct signaling center and progenitor identity dynamics initiate human forebrain patterning
Article Snippet: .. Briefly, dissected samples were chopped into smaller pieces (depending on size) and incubated in FACSmax (Amsbio, T200100) cell dissociation solution containing up to 300 U/mL Papain (Roche, 10108014001) at 37 ° C for 10-15 min, depending on size of pieces. .. After removal of dissection solution, an HBSS solution with 5% FBS, 10 μ M Rock inhibitor (Stemcell Technologies, Y-27632), and 1X non-essential amino acids (ThermoFisher Scientific, 11140035) was added, and cells were disaggregated by repeated pipetting (up to 20 times).

Flow Cytometry:

Article Title: Fgfr3 Is a Positive Regulator of Osteoblast Expansion and Differentiation During Zebrafish Skull Vault Development.
Article Snippet: The solution was strained using a 40 μm cell strainer (Corning), and the cells were washed in α-MEM supplement with 10% FBS. .. Prior to flow cytometry, the cells were suspended in FacsMax (amsbio) and stained with SytoxTM green nucleic acid stain (1:100000, Invitrogen) and Hoechst (1:5000, Thermo Fisher). .. To sort the cells, beads (Sony-LE-B3001) were used to select events larger than 3 μm.

Staining:

Centrifugation:

Article Title: Neanderthal-derived variants increase SOX9 enhancer activity in craniofacial progenitors that shape jaw development
Article Snippet: .. The samples were washed twice using 0.5× Danieau's solution and once with FACSmax (Amsbio) after centrifugation at 300 g for 1 min at 4°C. ..

Article Title: Neanderthal-derived variants increase SOX9 enhancer activity in craniofacial progenitors that shape jaw development.
Article Snippet: .. The samples were washed twice using 0.5× Danieau’s solution and once with FACSmax (Amsbio) after centrifugation at 300 g for 1 min at 4°C. ..

Concentration Assay:

Article Title: A method to investigate muscle target-specific transcriptional signatures of single motor neurons.
Article Snippet: Therefore, a mild papain dissociation method was performed, and dissociated cells were plated in a neuron-specific media (plating media: Neurobasal Gibco, 5% fetal bovine serum [FBS] and 1% GlutaMAX Gibco).25 One hour and a half before the end of the incubation period required for axonal tracing, we put the Neurobasal plating media in a Petri dish inside a cell culture incubator for CO2 equilibration. .. Additionally, half an hour before the end, we mixed FACSmax (Amsbio) dissociation media with papain (Roche) to get a 0.25 mg/mL final concentration of the enzyme. ..

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